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Our LML IHC Markers

Epitope target @ LML vs.
ref. lab		 FIXATIVE Vendor. CAT.# Ab/type(CLONE)/
animal/
detection		 TITER type
unmask		 FFPE/FRZ
FIXATIVE		 STAINING
PATTERN		 TARGET Positive
Control
A-1-ACT LML NBF Dako A0022 Poly 1:6400 PROT Y/N 10%NBF Cytoplasmic Histiocytes breast epi.; tonsil
ACTH   NBF             Cytoplasmic   pituitary
AFP LML NBF Dako A0008 Poly   RV Y/Y 10%NBF Cytoplasmic Hepatocytes Fetal Liver; HCC
ALK-1 protein   NBF             Cyto &/or nuclear anaplastic large cell lymphoma cells; PSFMT Hodgkin or ALCL tissue
AMACR (see P504S) ref                    
pan-B-cell (CD20) LML NBF Zymed 18-0088 L-26 1:100 Borg Y/Y 10%NBF Cytoplasmic B-cells Tonsil
bcl-1 (see cyclin D1) LML NBF             nuclear mantel cell lymphoma marker tonsilar epithelial basal & parabasal epithelial nuclei; melanoma cell nuclei
bcl-2 LML M2 Zymed 18-0193 Bcl-2/100/D5 1:400 Borg Y/Y 10%NBF BEST: Bouin's or B5 or M2 Memb & cyto normal & reactive germinal centers, negative; neoplastic follicles/nodules, positive[also] Tonsil; normal melanocytes
bcl-6   NBF             nuclear    
beta-catenin LML NBF Dako M3539 beta-catenin-1 monomouse       cytoplasmic toward membrane & may be nuclear adhesion junct. protein subunit of cadherin & commonmolecular CRC is pos. (along w/ p53) fibroblasts and endothelial cells
Brst-2 (GCDFP-15) LML NBF Biocare PM113AA D6   RV Y/N 10%NBF Cytoplasmic apocrine differentiation, breast cancer, axillary sweat glands and submandibular salivary glands Normal axilla apocrine, known pos. breast ca., & bronchial glands.
Ber-H2 (see CD30) LML NBF               embryonal ca.; Reed-Sternberg cells  
Calcitonin LML NBF  NC NCL-CALp Poly 1:200 RV Y/Y 10%NBF Cytoplasmic C-cells of Thyroid Thyroid
Calretinin LML NBF (alcohol reduces) Zymed 08-42111 Poly Pre RV Y/Y 10%NBF Cytoplasmic & nuclear Mesothelium; basal-type breast cancer Normal cerebral cortex, pleura & mesothelium (appendix & fal. tube), and mesothelioma; testis (Leydig cells positive); adrenal adenoma
CD3 (pan-T-Cell) LML   Zymed 804478 Poly PRE Borg Y/N 10%NBF Membrane T-cell membrane (essentially a pan-T cell) Tonsil
CD4     Ventana 250-2712 1F6 PRE Borg 15min Y/N 10%NBF Membrane T-helper cells Tonsil
CD5 LML B5/mercuric decrease it3; OK with bone marrow decal NC NCL-CD5-4C7 4C7 1:30 Borg Y/N 10%NBF, Bouin's, B5 Membrane T-cells in mantle cell lymphoma & CLL/SLL; 95% of thymocytes & about 75% of periph. bllod lymphocytes Tonsil
CD8     Ventana 250-2714 1A5 PRE Borg Y/N 10%NBF Membrane T-suppressor cells Tonsil
CD10 LML NBF NC NCL-CD10-270 270 1:50 Borg Y/Y 10%NBF Membrane; brush border CLL, FCC, CALLA, lymphs, Burkitt's, RCC Tonsil
CD15 (Leu-M1) LML NBF Zymed Leu-M1 LEU-M1 PRE BORG Y/N 10%NBF, Bouin's, B5 Membrane (memb. plus Golgi in R-S cells) Granulocytes [internal pos. auto-control]; Reed-Sternberg Tonsil/LN
CD20 (see pan-B) LML NBF             cytoplasmic B-lymphs Tonsil
CD23     NC NCL-CD23-1B12 1B12 1:80 Borg Y/Y 10%NBF Membrane B-lymphs, CLL/SLL Tonsil
CD30 (Ki-1; Ber-H2) LML NBF Zymed 18-0155 Ber-H2 1:20 RV Y/N 10%NBF Membrane (memb. plus Golgi in R-S cells) Activated T&B cells, NK cells, R-S Cells, ALCL cells, adenoca., embryonal ca.; [internal pos. auto-control]___ Tonsil, LN
CD34 LML   CM CMA-335 GBEND/10 PRE Borg Y/Y 10%NBF Membrane Hematopoietic progenitor cells, myeloid and lymphoid lineage, and vascular endothelium Tonsil
CD45(see LCA)                 Membrane    
CD45R0 (T cell)                   pan-T cell  
CD56 LML   BC CM164B BC56CO4 1:80 RV Y/N 10%NBF Membrane very sensitive neuroendocrine marker; NCAM, Skin, SCC, Neurons, Astrocytes, NK cells, Activated T-cells Pancreas, neuroblastoma
CD68 LML NBF BC CM033B KP1 1:1280 RV Y/Y 10%NBF Cytoplasmic Myeloid Leuk,macrophage, monocytes, Kupffer cells Tonsil
CD99     CD99 M3601 12E7       membranous & cytoplasmic in ES & PNET & cytoplasmic in others Ewing Sarcomas, neuroectodermal tumors (PNET), peripheral neuroepitheliomas, lymphoblastic lymphoma & leukemia & others tonsil basal & parabasal squamous has a distinct membrane pos. when process is optimal; pancreatic islet cells, Sertoli cells, cortical thymocytes, and granulosa cells
CD117 (c-kit) LML               CD117 is c-kit proto-oncogene transmembrane glycoprotein product. mast cells, melanocytes. GIST normal breast epithelium
CD138 LML               Cytoplasmic plasma cells tonsil
CDX-2 ref NBF             nuclear TF colon cancer nl colon epi.
CEA (COL-1) LML   Zymed 18-0057 COL-1 monoclonal 1:300 RV Y/Y 10%NBF memb/ cytopl CEA in G.I. intestinal tumors & does not react with normal colon Colon Ca
mCEA LML    Ventana 760-2507 TF-3H8-1 monoclonal PRE RV Y/N 10%NBF Membrane and Cytoplasmic Epithelial Cells CAM
pCEA LML   Dako A 0115 polyclonal       HCC canalicular stains HCC with canalicular pattern which can be weak in less diff. HCCs; stains met. adenoca.; 100% med. TC colon ca
Chromogranin A LML   Ventana 760-2519 LK2H10 PRE Borg Y/N 10%NBF memb/cytopl Secretory granules of NE cells...epithelial Pancreas
c-kit  (see CD117) LML                    
ck5/6                 cytoplasmic, fibrillar mesothelial & mesothelioma marker; "basal like" breast cancer marker; squamous & transitional cell marker tonsil
CK-7 LML    Dako M7018 OV-TL 1:200 None Y/Y 10%NBF cytoplasmic, fibrillar non-SCC ca. above diaphragm; transitional epithelia about 100% Breast
CK-8 (LMWK) LML    Dako M0631 35BH11 1:200 Prot-1 Y/Y 10%NBF BEST: Carnoy's or Methacarn cytoplasmic, fibrillar NS epithelium Breast
CK-19 ref NBF             cytoplasmic, fibrillar classical PTC  
CK-20 LML   Dako M7019 Ks20.8 1:200 None Y/N 10%NBF cytoplasmic, fibrillar non-SCC ca. below diaphragm; transitional 40-70% CAM, colon
CMV                      
collagen type IV                 extracellular sarcomas pos. appendix
cyclin D1(bcl-1) LML NBF             nuclear MCL; melanoma more likely when lots nuclei pos. at many levels in atypia vs. MIS breast ca.; melanoma cells
Desmin LML   Dako M0760 D33 1:50 RV Y/N 10%NBF Membrane Intermediate filaments in striated and smooth muscle cells CAM; appendix
E-cadherin LML   Zymed 18-0223 4A2C7 1:400 Borg Y/Y 10%NBF Membrane Tissue morphogenesis and structure, osteoblasts, PN Schwann cell, Langerhan's cells ductal ca. breast
EGFR (HER-1) LML NBF           10% NBF; & works /w Hartmann's membrane basal-like breast ca. SC junct. cervix; villous trophoblasts of placenta; epidermis
EMA LML   Ventana 250-2508 Mc5 PRE RV Y/N 10%NBF Membrane/ cytoplasmic Normal epithelial cells; RCC breast
ER LML NBF (alcohol reduces) Ventana 760-2596 6F11 Pre Borg Y/N 10%NBF Nuclear (transcription factor) Lobular, tubular, & luminal breast ca's rich with PR & implies responsiveness to hormonal blockade TX breast; uterus
Factor VIII (F-8) LML   Dako N1505 Vwf 1:500 RV Y/N 10%NBF Cytoplasmic Vascular endothelial cells Tonsil
Fascin   NBF Cell Marque   55k-2         dendritic cells;  Hodgkin lymphoma cells (R-S); [internal pos. auto-control] is endothelium & dendritic cells in benign lymphoid follicles Hodgkin with known R-S cells
FSH                      
GCDFP-15 (see Brst-2) LML                  about 50% of breast cancers (but not "basal like") are positive, especially if apocrine change (only 5% of medullary); sweat gland & salivary gland pos. Breast
GFAP LML   Ventana 250-2516 Poly PRE None Y/N 10%NBF Cytoplasmic astrocytes, ependymal cells, activated Ito cells, activated myofibroblasts Brain
gp100 (see HMB-45)         HMB-45            
HCG LML   NC NCL-HCGp Poly 1:500 RV Y/Y 10%NBF Cytoplasmic Placenta trophoblasts; Note: cross-reacts weakly with LH, FSH and TSH Placenta
Hep Par-1 LML   CM   OCH1E5
(hepatocyte specific antigen: HSA)		       granular cytoplasmic >90% sens. for B9 or malig. hepatocytes...liver cells; also pos. in some gastric, ovarian, and neuroendocrine ca. liver
Helicobacter pylori LML   CM CMC435 Poly 1:50 RV Y/Y 10% NBF , M2 Surface & cytoplasm HBP in epithelial cells GIBX
HER-1 (see EGFR)                      
HER-2 LML   Dako A0485 c-erb-2 1:1000 Borg Y/Y 10%NBF, Bouin's Membrane product of the HER-2/neu oncogene Epithelial cells HER-2 pos. breast
HGH                      
HMB-45 LML NBF Dako M0634 HMB-45 1:200 Borg Y/Y 10%NBF Membrane Melanomas; renal AML Melanoma
HSVI/II                      
K-903 (HMWK, squamous) (ck 1,5,10,14) LML NBF; M2 Dako M0630 34BE12
(reactivity fades with prolonged NBF fixation time1)		 1:40 Prot-1 Y/Y 10%NBF BEST: Carnoy's or Methacarn Membrane prostate basal; squamous epithelia & SCC  Prostate (ME cells pos.)
Kappa light chains LML NBF
(not mercuric)		 Dako M0827 R10-21-F3 1:80 RV Y/Y 10%NBF Coarse meshwork pattern Plasma cells and B-cells Tonsil
keratin, HMW (see keratin 903)
 LML NBF             cytoplasmic, fibrillar basal cells & positivity almost always indicates benign the normal glands in the cores
keratin, LMW (see ck8) LML               cytoplasmic, fibrillar    
Ki-1 (see CD30) LML                     
Ki-67 LML markedly reduced w/ the common glyoxyl-based formalin substitutes3 Dako M7240 mono(MIB-1) 1:40 Borg Y/Y 10%NBF, Bouin's, B5, Zenker's; Hartmann's Nuclear Active Phase cells (G1,S, G2 and M) Tonsil
Lambda light chains LML NBF
(not mercuric)		 Dako M0614 N10/2 1:80 RV Y/Y 10%NBF Membrane Plasma cells Tonsil
LCA (CD45) LML   Ventana 250-2136 RP2/18 PRE RV Y/N 10%NBF Membrane leukocytic cell lineage & if pos. is nearly 100% specific Tonsil
Leu-M1 (see CD15) LML NBF                  
LH                      
Lysozyme     NC NCl-Muram Poly 1:200 RV Y/N 10%NBF Cytoplasmic Myeloid/monocytic cells Tonsil, Colon, Spleen
Mart-1 (Melan-A) LML   Dako M7196 A103 1:40 Borg Y/Y 10%NBF Membrane Melan A mRNA+ cells, melanocytes, adrenal cortical ad/ca Melanoma
MBP     Ventana 250-2791 Poly PRE/Lyph RV Y/N 10%NBF   CNS and PNS soft tissue tumors Brain, kidney, spinal cord
Melan A (see Mart-1) LML                    
MSA (muscle specific actin)     Ventana 250-2790 HHF-35 PRE/Lyph RV Y/N 10%NBF Cytoplasmic Skeletal, cardiac, and smooth muscle cells; rhabdomyoblasts CAM
Myeloperoxidase (MPO)     NC NCL-MYELOp Poly 1:60 RV Y/Y 10%NBF Cytoplasmic Myeloid Cells Tonsil
muscle actin (MA)                      
myoglobin                       
neurofilament (NFP) LML   CM CMA518-520 2F11         neural tumors, pheos, carcinoids, neuroendocrine ca. of skin & lung  
NSE LML   Ventana 250-2517 BBS/NC/V1-H14 PRE RV Y/N 10%NBF Cytoplasmic Neurons, NE cells, T-cells Pancreas
Pan-keratin LML   Dako M3515 AE1/AE3 (cocktail) 1:40 Prot-1 Y/Y 10%NBF Membrane Stratified squamous epithelia (basic and acidic keratins) Breast
Pan-melanoma LML   VP-Echelon VP165G5 HMB45, MART-1(DT101 & BC199), & tyrosinase (T311) cocktail           melanoma
PAX2 ref               nuclear RCC  
PAX-5 LML NBF Ventana 760-4270 24            
p53 LML NBF (alcohol can reduce it so don't use Hartmann's block) Ventana 790-2912 DO-7     10% NBF or Hartman's (LMC-03-4613), but if that block is negative it may be false neg. nuclear normal p53 "guards" the genome from damage & brief half-life; mutation prolongs half life so IHC positivity implies mutation & increase helps define common CRC & non-luminal-A breast ca. common colon ca.
P504S ref                 prostate cancer cells pos.  
p63 LML   NBF (alcohol reduces) Dako N1604 4A4 monomouse       nuclear    
PLAP LML NBF Dako M7191 8A9 monomouse 1:50 RV Y/Y 10%NBF Membrane Syncytiotrophoblasts Placenta, seminoma
placental lactogen LML NBF Dako A0137 PL polyrabbit            
PR LML NBF (alcohol reduces) Ventana 760-2816 16 Pre Borg Y/N 10%NBF Nuclear (transcription factor) Lobular, tubular, & luminal breast ca's rich with PR & implies responsiveness to homornal blockade TX Breast Ca
prolactin LML NBF Dako   polyrabbit            
PSA LML NBF Dako   polyrabbit PRE RV Y/N 10%NBF Membrane/ cytoplasmic Normal prostatic epithelial cells Prostate
racemase (see P504S) ref NBF                  
S-100 LML NBF Dako N1573 polyrabbit PRE Borg Y/N 10%NBF Homogeneous cytoplasmic & nuclear NE Cells Melanoma or peripheral nerves
smooth muscle actin LML NBF Dako N1584 1A4 monomouse            
SMM-hc LML NBF Dako M3558 SMMS-1monomouse 1:50 Borg Y/Y 10%NBF Cytoplasmic smooth muscle (& myoepithelial & pericytes & myofibroblasts) Breast
surfactant ApoA ref               Cytopasmic   pneumocytes
Synaptophysin LML NBF Ventana (CM) 250-2668 Poly PRE RV Y/Y 10%NBF Cytoplasmic NE Cells...neural Pancreas
TAG-72 LML NBF Vetana                
T-cell (CD45RO) LML   Zymed 18-0112 A6 1:100 Borg Y/Y 10%NBF Membrane T-cells Tonsil
TdT LML NBF (alcohol reduces)             nuclear    
Thrombomodulin LML                   Placenta
Thyroglobulin LML   Ventana 250-2671 2H11/6E1 PRE RV Y/N 10%NBF homogeneous cytoplasmic Langerhan cells, thyroid cells; anaplastic TC only rare pos cells Thyroid
TTF-1 LML NBF (alcohol reduces...so watch out for false neg. in effusions) Zymed 18-0221 8G7G3/1 1:200 Borg Y/Y 10%NBF (TFs adversely affected by alcohol) Nuclear (transcription factor) epithelial cells of the lung (pneumocytes) and thyroid follicle cells, including neuroendocrine...but some NE of other sites are positive (e. g., prostate2) Lung/thryroid
TRAP LML                    
ubiquitin LML                 Mallory bodies  
villin                 apical membrane brush border pattern is highly specific for GI tract adenoca.  
Vimentin LML   Ventana 250-2754 V9 PRE/Lyph None Y/N 10%NBF Cytoplasmic melanoma, sarcoma, spindled & poorly/undiff. ca., RCC, thyr. ca. intestines, appendix, CAM

References:

  1. Varma M & Jasani B, "Diagnostic Utility of Immunohistochemistry In Morphologically Difficult Prostate Cancer: A Review of Current Literature", Histopathology 47(1):1-16, 2005.
  2. True LD, CME course, 1 May 2005, 12th Annual SP Seminar, Pittsburgh .
  3. Yaziji H & Barry T, "Diagnostic Immunohistochemistry: What can Go Wrong", Adv. Anat. Pathol., 13(5):238-246, Sept. 2006.

(posted April 2002; latest addition 6 March 2010)